Effects of Transport and Holding Stress on Prussian Carp (<i>Carassius gibelio</i>, Bloch, 1782.) Leukogram Pattern

Abstract Stress inevitably occurs during any fish handling and manipulation in culturing, research, or clinical examination situations that require capture removal of from water. Different stress factors can affect the changes relative numbers function cells immune system. Catching, transportation over-crowding caused stress-induced total number leukocytes thrombocytes, as well leukocyte formula Prussian carp ( Carassius gibelio , Bloch, 1782) presented here. Cytochemical characterization cell types was performed by applying Myeloperoxidase (MPO), Periodic Acid Schiff (PAS) Sudan Black B (SBB) staining blood smears. is a rapid efficient method for white differentiation insight their functional status. Comparison analysis hematological parameters with without exposure to stressful conditions such capture, manipulation, transport holding, revealed significant differences between stressed non-stressed fish. Significant reduction thrombocytes lymphocytes increase neutrophil count were observed animals. However, monocytes not observed. Deviations estimated reference intervals clearly indicated presence/absence reaction some extent its intensity. Estimated morphological cytochemical appearance form solid basis further research cellular hematology carp.